Coding

Part:BBa_K3317013

Designed by: erick hernandez   Group: iGEM19_UANL   (2019-10-18)


pduU protein from S. enterica LT2

Propanediol utilization protein PduU from S. enterica LT2 (NC_003197.1)from position 7 to 46 it has a linker region that contains the ribosomal restart site, this sequence is contained in plasmid pMCY30 generously provided by Dr. Mimi C. Yung. A SpeI site and a NheI site flank the sequence for excision of pduU if necessary as described by Yung, M. C et. to 2017 this piece is part of a set which forms the BMCs (BBa_K3317033)


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1
    Illegal PstI site found at 161
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 398
    Illegal SpeI site found at 1
    Illegal PstI site found at 161
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 139
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1
    Illegal PstI site found at 161
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1
    Illegal PstI site found at 161
    Illegal AgeI site found at 281
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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